Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

doi: 10.1155/2021/6623464

Figure Lengend Snippet: Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.

Article Snippet: For Hoechst staining, 10 μ L Hoechst live cell staining solution (Beyotime Institute of Biotechnology) was added to the culture medium and mixed gently.

Techniques: Flow Cytometry, Double Staining, Control, Staining, Western Blot

Journal: Cell death & disease

Article Title: Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharide.

doi: 10.1038/s41419-023-06318-6

Figure Lengend Snippet: Fig. 2 hMDMs exposed to CSE and LPS display apoptotic hallmarks. Representative images of hMDMs treated with 1 μg/ml of LPS and 20% CSE, alone or in combination, for 24 h and stained with (A) Hoechst 33342 for nuclear morphology evaluation and (B) JC-1 dye for MMP assessment. C Quantification of Hoechst 33342 intensity expressed as mean fluorescent intensity (MFI). D Quantification of the red (485/ 590 nm)/green (485/529 nm) fluorescence intensity ratio after JC1 staining. Scale bar: 50 μm. Data are presented as mean ± SEM (N = 3 independent donors).

Article Snippet: Hoechst fluorescent nucleic acid stain (33342) was obtained from ImmunoChemistry technology, Davis, California, USA.

Techniques: Staining